Journal: American Journal of Cancer Research

Article Title: Downregulation of cyclin-dependent kinase 8 by microRNA-148a suppresses proliferation and invasiveness of papillary thyroid carcinomas

doi:

Figure Lengend Snippet: miR-148a targets CDK8 expression. A. Putative binding site of miR-148a in the CDK8 3’-UTR as predicted by TargetScan. B. Dual-luciferase activity in WT and mutant CDK8 3’-UTR reporter constructs, relative to miR-C, in the presence of miR-148a. C. Expression of CDK8 as detected through western blots in TPC-1 and K1 cells transfected with either miR-148a mimics or miR-C. **P < 0.05.

Article Snippet: The sections were then incubated with rabbit anti-CDK8 (Abgent, San Diego, CA) in a humidified chamber overnight at 4°C.

Techniques: Expressing, Binding Assay, Luciferase, Activity Assay, Mutagenesis, Construct, Western Blot, Transfection

Journal: American Journal of Cancer Research

Article Title: Downregulation of cyclin-dependent kinase 8 by microRNA-148a suppresses proliferation and invasiveness of papillary thyroid carcinomas

doi:

Figure Lengend Snippet: Effects of CDK8 inhibition in PTC cells with upregulated miR-148a expression. A. Detection of CDK8 expression levels through immunohistochemistry. B. Detection of CDK8 expression levels through western blotting for each group of transfected TPC-1 and K1 cells. C-F. Cell proliferation measurements by MTT assays, migration capacity measurements by colony formation assays, and invasion capacity measurements by transwell assays. *P < 0.05, **P < 0.05.

Article Snippet: The sections were then incubated with rabbit anti-CDK8 (Abgent, San Diego, CA) in a humidified chamber overnight at 4°C.

Techniques: Inhibition, Expressing, Immunohistochemistry, Western Blot, Transfection, Migration